1n2c Summary

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NITROGENASE COMPLEX FROM AZOTOBACTER VINELANDII STABILIZED BY ADP-TETRAFLUOROALUMINATE

The structure was published by Schindelin, H., Kisker, C., Schlessman, J.L., Howard, J.B., and Rees, D.C., in 1997 in a paper entitled "Structure of ADP x AIF4(-)-stabilized nitrogenase complex and its implications for signal transduction." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 1997.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely NITROGENASE MOLYBDENUM-IRON PROTEIN and NITROGENASE IRON PROTEIN.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterooctamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A NITROGENASE MOLYBDENUM-IRON PROTEIN P07328 (2-492) (NIFD_AZOVI)search Azotobacter vinelandiisearch 100% 491 97%
C NITROGENASE MOLYBDENUM-IRON PROTEIN P07328 (2-492) (NIFD_AZOVI)search Azotobacter vinelandiisearch 100% 491 97%
B NITROGENASE MOLYBDENUM-IRON PROTEIN P07329 (2-523) (NIFK_AZOVI)search Azotobacter vinelandiisearch 100% 522 100%
D NITROGENASE MOLYBDENUM-IRON PROTEIN P07329 (2-523) (NIFK_AZOVI)search Azotobacter vinelandiisearch 100% 522 100%
E NITROGENASE IRON PROTEIN P00459 (2-290) (NIFH1_AZOVI)search Azotobacter vinelandiisearch 93% 289 94%
F NITROGENASE IRON PROTEIN P00459 (2-290) (NIFH1_AZOVI)search Azotobacter vinelandiisearch 93% 289 94%
G NITROGENASE IRON PROTEIN P00459 (2-290) (NIFH1_AZOVI)search Azotobacter vinelandiisearch 93% 289 94%
H NITROGENASE IRON PROTEIN P00459 (2-290) (NIFH1_AZOVI)search Azotobacter vinelandiisearch 93% 289 94%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P07328 (2 - 492) NITROGENASE MOLYBDENUM-IRON PROTEIN Azotobacter vinelandii
P07329 (2 - 523) NITROGENASE MOLYBDENUM-IRON PROTEIN Azotobacter vinelandii
P00459 (2 - 290) NITROGENASE IRON PROTEIN Azotobacter vinelandii

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P07328) Nitrogenase iron-molybdenum proteinsearch Nitrogenase molybdenum iron protein domainsearch PF00148: Nitrogenase component 1 type Oxidoreductasesearch
B, D (P07329) Nitrogenase iron-molybdenum proteinsearch Nitrogenase molybdenum iron protein domainsearch, Nitrogenase Molybdenum-iron Protein, subunit B, domain 4search PF00148: Nitrogenase component 1 type Oxidoreductasesearch, PF11844: Domain of unknown function (DUF3364)search
E, F, G, H (P00459) Nitrogenase iron protein-likesearch P-loop containing nucleotide triphosphate hydrolasessearch PF00142: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC familysearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P07328) nitrogenase activitysearch carbonyl sulfide nitrogenase activitysearch ATP bindingsearch metal ion bindingsearch oxidoreductase activitysearch iron-sulfur cluster bindingsearch nucleotide bindingsearch oxidation-reduction processsearch nitrogen fixationsearch molybdenum-iron nitrogenase complexsearch
B, D (P07329) iron-sulfur cluster bindingsearch nitrogenase activitysearch oxidoreductase activitysearch metal ion bindingsearch carbonyl sulfide nitrogenase activitysearch nucleotide bindingsearch ATP bindingsearch oxidation-reduction processsearch nitrogen fixationsearch molybdenum-iron nitrogenase complexsearch
E, F, G, H (P00459) oxidoreductase activitysearch iron-sulfur cluster bindingsearch ATP bindingsearch nitrogenase activitysearch 4 iron, 4 sulfur cluster bindingsearch nucleotide bindingsearch metal ion bindingsearch carbonyl sulfide nitrogenase activitysearch oxidation-reduction processsearch nitrogen fixationsearch molybdenum-iron nitrogenase complexsearch

Chain InterPro annotation
A, C Nitrogenase component 1, conserved sitesearch Nitrogenase/oxidoreductase, component 1search Nitrogenase molybdenum-iron protein alpha chainsearch Nitrogenase component 1, alpha chainsearch
B, D Nitrogenase component 1, conserved sitesearch Nitrogenase/oxidoreductase, component 1search Nitrogenase molybdenum-iron protein beta chainsearch Nitrogenase molybdenum-iron protein beta chain, N-terminalsearch
E, F, G, H Nitrogenase iron protein, subunit NifH/Protochlorophyllide reductase, subunit ChlLsearch Nitrogenase iron protein NifHsearch P-loop containing nucleoside triphosphate hydrolasesearch