› 1n25

X-ray diffraction
2.8Å resolution

Crystal structure of the SV40 Large T antigen helicase domain

Released:
DOI: 10.2210/pdb1n25/pdb
PDB entry 1n25 coloured by chain, front view.
PDB entry 1n25 coloured by chain, side view.
PDB entry 1n25 coloured by chain, top view.
Source organism: Macaca mulatta polyomavirus 1
Primary publication:
Structure of the replicative helicase of the oncoprotein SV40 large tumour antigen.
Li D, Zhao R, Lilyestrom W, Gai D, Zhang R, DeCaprio JA, Fanning E, Jochimiak A, Szakonyi G, Chen XS
Nature 423 512-8 (2003)
PMID: 12774115

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large T antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 42.5 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03070 (Residues: 260-627; Coverage: 52%)
Sequence domains: Polyomavirus large T antigen C-terminus
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P321
Unit cell:
a: 120.321Å b: 120.321Å c: 132.202Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.243 0.276
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Evolutionary relationships and structural mechanisms of AAA+ proteins.
Erzberger et al. (2006)
50 more

14 mentions without citation

Understanding helicases as a means of virus control.
Frick et al. (2006)
13 more