› 1n1t

X-ray diffraction
1.6Å resolution

Trypanosoma rangeli sialidase in complex with DANA at 1.6 A

Released:
DOI: 10.2210/pdb1n1t/pdb
PDB entry 1n1t coloured by chain, front view.
PDB entry 1n1t coloured by chain, side view.
PDB entry 1n1t coloured by chain, top view.
Source organism: Trypanosoma rangeli
Primary publication:
The high resolution structures of free and inhibitor-bound Trypanosoma rangeli sialidase and its comparison with T. cruzi trans-sialidase.
Amaya MF, Buschiazzo A, Nguyen T, Alzari PM
J Mol Biol 325 773-84 (2003)
PMID: 12507479

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 641 amino acids
Theoretical weight: 69.91 KDa
Source organism: Trypanosoma rangeli
Expression system: Escherichia coli
UniProt:
  • Canonical: O44049 (Residues: 23-660; Coverage: 100%)
Sequence domains: BNR repeat-like domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand DAN 1 x DAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 73.945Å b: 96.258Å c: 106.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.197
Expression system: Escherichia coli

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Citations

5 review citations

Diversity of microbial sialic acid metabolism.
Vimr et al. (2004)
4 more

6 mentions without citation

Genetic structure and expression of the surface glycoprotein GP82, the main adhesin of Trypanosoma cruzi metacyclic trypomastigotes.
Correa et al. (2013)
5 more