› 1ms7

X-ray diffraction
1.97Å resolution

X-ray structure of the GluR2 ligand-binding core (S1S2J) in complex with (S)-Des-Me-AMPA at 1.97 A resolution, Crystallization in the presence of zinc acetate

Released:
DOI: 10.2210/pdb1ms7/pdb
PDB entry 1ms7 coloured by chain, front view.
PDB entry 1ms7 coloured by chain, side view.
PDB entry 1ms7 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
GluR2 ligand-binding core complexes: importance of the isoxazolol moiety and 5-substituent for the binding mode of AMPA-type agonists.
Kasper C, Lunn ML, Liljefors T, Gouaux E, Egebjerg J, Kastrup JS
FEBS Lett 531 173-8 (2002)
PMID: 12417307

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 263 amino acids
Theoretical weight: 29.22 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand SHI 3 x SHI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21212
Unit cell:
a: 113.723Å b: 162.863Å c: 47.133Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Competitive AMPA receptor antagonists.
Catarzi et al. (2007)
6 more

1 mention without citation

Benchmark Sets for Binding Hot Spot Identification in Fragment-Based Ligand Discovery.
Wakefield et al. (2020)