1mr1

X-ray diffraction
2.85Å resolution

Crystal Structure of a Smad4-Ski Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-146309 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 25.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13485 (Residues: 319-552; Coverage: 42%)
Gene names: DPC4, MADH4, SMAD4
Sequence domains: MH2 domain
Structure domains: Tumour Suppressor Smad4
Ski oncogene Chains: C, D
Molecule details ›
Chains: C, D
Length: 99 amino acids
Theoretical weight: 11.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12755 (Residues: 219-313; Coverage: 13%)
Gene name: SKI
Sequence domains: c-SKI Smad4 binding domain
Structure domains: SAND domain-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 109.8Å b: 109.8Å c: 141.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.231 0.28
Expression system: Escherichia coli