› 1mox

X-ray diffraction
2.5Å resolution

Crystal Structure of Human Epidermal Growth Factor Receptor (residues 1-501) in complex with TGF-alpha

Released:
DOI: 10.2210/pdb1mox/pdb
PDB entry 1mox coloured by chain, front view.
PDB entry 1mox coloured by chain, side view.
PDB entry 1mox coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a truncated epidermal growth factor receptor extracellular domain bound to transforming growth factor alpha.
Garrett TP, McKern NM, Lou M, Elleman TC, Adams TE, Lovrecz GO, Zhu HJ, Walker F, Frenkel MJ, Hoyne PA, Jorissen RN, Nice EC, Burgess AW, Ward CW
Cell 110 763-73 (2002)
PMID: 12297049

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132704 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Epidermal growth factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 501 amino acids
Theoretical weight: 55.69 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00533 (Residues: 25-525; Coverage: 42%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains:
Structure domains:
Transforming growth factor alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 50 amino acids
Theoretical weight: 5.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01135 (Residues: 40-89; Coverage: 37%)
Gene name: TGFA
Structure domains: Laminin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand PT 7 x PT
Ligand CD 11 x CD
Ligand CL 4 x CL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 51.59Å b: 198.71Å c: 78.9Å
α: 90° β: 102.03° γ: 90°
R-values:
R R work R free
0.24 0.237 0.289
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

113 review citations

ERBB receptors and cancer: the complexity of targeted inhibitors.
Hynes et al. (2005)
112 more

36 mentions without citation

Structure-based view of epidermal growth factor receptor regulation.
Ferguson KM. (2008)
35 more