› 1mjt

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF SANOS, A BACTERIAL NITRIC OXIDE SYNTHASE OXYGENASE PROTEIN, IN COMPLEX WITH NAD+ AND SEITU

Released:
DOI: 10.2210/pdb1mjt/pdb
PDB entry 1mjt coloured by chain, front view.
PDB entry 1mjt coloured by chain, side view.
PDB entry 1mjt coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structure of SANOS, a bacterial nitric oxide synthase oxygenase protein from Staphylococcus aureus.
Bird LE, Ren J, Zhang J, Foxwell N, Hawkins AR, Charles IG, Stammers DK
Structure 10 1687-96 (2002)
PMID: 12467576

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 reduced flavodoxin + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 oxidized flavodoxin + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Nitric oxide synthase oxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 40.51 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A004 (Residues: 2-346; Coverage: 96%)
Gene name: nos
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD

Cofactor: Ligand HEM 2 x HEM
Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand ITU 2 x ITU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 65.78Å b: 115.14Å c: 126.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.182 0.241
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Update on mechanism and catalytic regulation in the NO synthases.
Stuehr et al. (2004)
11 more

5 mentions without citation

Phenolic Content and Antimicrobial and Anti-Inflammatory Effects of Solidago virga-aurea, Phyllanthus niruri, Epilobium angustifolium, Peumus boldus, and Ononis spinosa Extracts.
Ferrante et al. (2020)
4 more