› 1mi8

X-ray diffraction
2Å resolution

2.0 Angstrom crystal structure of a DnaB intein from Synechocystis sp. PCC 6803

Released:
DOI: 10.2210/pdb1mi8/pdb
PDB entry 1mi8 coloured by chain, front view.
PDB entry 1mi8 coloured by chain, side view.
PDB entry 1mi8 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing.
Ding Y, Xu MQ, Ghosh I, Chen X, Ferrandon S, Lesage G, Rao Z
J Biol Chem 278 39133-42 (2003)
PMID: 12878593

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicative DNA helicase, 1st part Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.6 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55418 (Residues: 379-478, 500-501; Coverage: 12%)
Gene names: dnaB, slr0833
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 58.17Å b: 58.17Å c: 70.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.21 0.263
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Protein splicing in cis and in trans.
Saleh et al. (2006)
7 more

12 mentions without citation

Chemoenzymatic Semisynthesis of Proteins.
Thompson et al. (2020)
11 more