› 1mh4

X-ray diffraction
2.3Å resolution

maltose binding-a1 homeodomain protein chimera, crystal form II

Released:
DOI: 10.2210/pdb1mh4/pdb
PDB entry 1mh4 coloured by chain, front view.
PDB entry 1mh4 coloured by chain, side view.
PDB entry 1mh4 coloured by chain, top view.
Source organisms:
Primary publication:
Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera.
Ke A, Wolberger C
Protein Sci 12 306-12 (2003)
PMID: 12538894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein; Silenced mating-type protein A1 Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 46.4 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P0CY11 (Residues: 71-126; Coverage: 44%)
Gene names: HMRA1, JW3994, YCR097W, YCR97W, b4034, malE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 50.254Å b: 88.705Å c: 96.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.293
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Crystal structures of fusion proteins with large-affinity tags.
Smyth et al. (2003)
3 more

4 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
3 more