› 1mgy

X-ray diffraction
2Å resolution

Structure of the D85S mutant of bacteriorhodopsin with bromide bound

Released:
DOI: 10.2210/pdb1mgy/pdb
PDB entry 1mgy coloured by chain, front view.
PDB entry 1mgy coloured by chain, side view.
PDB entry 1mgy coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumping.
Facciotti MT, Cheung VS, Nguyen D, Rouhani S, Glaeser RM
Biophys J 85 451-8 (2003)
PMID: 12829500

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.9 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

4 bound ligands:
Ligand BR 2 x BR
Ligand K 1 x K
Ligand RET 1 x RET
Ligand LI1 8 x LI1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 52.498Å b: 121.326Å c: 73.875Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.236
Expression system: Halobacterium salinarum

Quick links

Citations

4 review citations

Bacteriorhodopsin: Would the real structural intermediates please stand up?
Wickstrand et al. (2015)
3 more

7 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
6 more