› 1mf1

X-ray diffraction
2.7Å resolution

Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with AMP

Released:
DOI: 10.2210/pdb1mf1/pdb
PDB entry 1mf1 coloured by chain, front view.
PDB entry 1mf1 coloured by chain, side view.
PDB entry 1mf1 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Feedback inhibition and product complexes of recombinant mouse muscle adenylosuccinate synthetase.
Iancu CV, Borza T, Fromm HJ, Honzatko RB
J Biol Chem 277 40536-43 (2002)
PMID: 12186864

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase isozyme 1 Chain: A
Molecule details ›
Chain: A
Length: 457 amino acids
Theoretical weight: 50.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28650 (Residues: 1-457; Coverage: 100%)
Gene names: Adss1, Adssl1
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 70.24Å b: 70.24Å c: 199.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.265
Expression system: Escherichia coli BL21

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Citations

7 citation in other articles

Molecular determinants for ATP-binding in proteins: a data mining and quantum chemical analysis.
Mao et al. (2004)
6 more