› 1m2v

X-ray diffraction
2.75Å resolution

Crystal Structure of the yeast Sec23/24 heterodimer

Released:
DOI: 10.2210/pdb1m2v/pdb
PDB entry 1m2v coloured by chain, front view.
PDB entry 1m2v coloured by chain, side view.
PDB entry 1m2v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat.
Bi X, Corpina RA, Goldberg J
Nature 419 271-7 (2002)
PMID: 12239560

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147303 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein transport protein SEC23 Chain: A
Molecule details ›
Chain: A
Length: 768 amino acids
Theoretical weight: 85.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15303 (Residues: 1-768; Coverage: 100%)
Gene names: P9705.14, SEC23, YPR181C
Sequence domains:
Structure domains:
Protein transport protein SEC24 Chain: B
Molecule details ›
Chain: B
Length: 926 amino acids
Theoretical weight: 103.73 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40482 (Residues: 1-926; Coverage: 100%)
Gene names: ANU1, SEC24, YIL109C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 90.309Å b: 126.371Å c: 180.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.257
Expression system: Escherichia coli

Quick links

Citations

96 review citations

GEFs and GAPs: critical elements in the control of small G proteins.
Bos et al. (2007)
95 more

9 mentions without citation

Complement and the multifaceted functions of VWA and integrin I domains.
Springer TA. (2006)
8 more