› 1m0v

Solution NMR

NMR STRUCTURE OF THE TYPE III SECRETORY DOMAIN OF YERSINIA YOPH COMPLEXED WITH THE SKAP-HOM PHOSPHO-PEPTIDE N-acetyl-DEpYDDPF-NH2

Released:
DOI: 10.2210/pdb1m0v/pdb
PDB entry 1m0v coloured by chain, ensemble of 20 models, front view.
PDB entry 1m0v coloured by chain, ensemble of 20 models, side view.
PDB entry 1m0v coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Solution structure and phosphopeptide binding to the N-terminal domain of Yersinia YopH: comparison with a crystal structure.
Khandelwal P, Keliikuli K, Smith CL, Saper MA, Zuiderweg ER
Biochemistry 41 11425-37 (2002)
PMID: 12234185

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140167 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase YopH Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 14.88 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08538 (Residues: 1-129; Coverage: 28%)
Gene names: pYV0094, yop2b, yopH
Sequence domains: YopH, N-terminal
Structure domains: Protein-tyrosine phosphatase, YopH, N-terminal domain
Src kinase-associated phosphoprotein 2 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q3UND0 (Residues: 73-79; Coverage: 2%)
Gene names: Prap, Ra70, Saps, Scap2, Skap2, Skap55r

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Yersinia outer proteins: role in modulation of host cell signaling responses and pathogenesis.
Viboud et al. (2005)
8 more

2 mentions without citation

A multi-pronged search for a common structural motif in the secretion signal of Salmonella enterica serovar Typhimurium type III effector proteins.
Buchko et al. (2010)
1 more