› 1m0o

X-ray diffraction
2.4Å resolution

Structure of Dialkylglycine Decarboxylase Complexed with 1-Amino-1-methylpropanephosphonate

Released:
DOI: 10.2210/pdb1m0o/pdb
PDB entry 1m0o coloured by chain, front view.
PDB entry 1m0o coloured by chain, side view.
PDB entry 1m0o coloured by chain, top view.
Source organism: Burkholderia cepacia
Primary publication:
Aminophosphonate inhibitors of dialkylglycine decarboxylase: structural basis for slow binding inhibition.
Liu W, Rogers CJ, Fisher AJ, Toney MD
Biochemistry 41 12320-8 (2002)
PMID: 12369820

Function and Biology Details

Reaction catalysed: 
2,2-dialkylglycine + pyruvate = dialkyl ketone + CO(2) + L-alanine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,2-dialkylglycine decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 46.58 KDa
Source organism: Burkholderia cepacia
Expression system: Escherichia coli
UniProt:
  • Canonical: P16932 (Residues: 1-433; Coverage: 100%)
Gene name: dgdA
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand K 1 x K
Ligand NA 1 x NA
Ligand MPM 1 x MPM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6422
Unit cell:
a: 152.84Å b: 152.84Å c: 86.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.144 0.139 0.201
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Glycosidase inhibition: assessing mimicry of the transition state.
Gloster et al. (2010)
4 more

2 mentions without citation

Flexible torsion-angle noncrystallographic symmetry restraints for improved macromolecular structure refinement.
Headd et al. (2014)
1 more