› 1lyv

X-ray diffraction
1.36Å resolution

High-resolution structure of the catalytically inactive yersinia tyrosine phosphatase C403A mutant in complex with phosphate.

Released:
DOI: 10.2210/pdb1lyv/pdb
PDB entry 1lyv coloured by chain, front view.
PDB entry 1lyv coloured by chain, side view.
PDB entry 1lyv coloured by chain, top view.
Source organism: Yersinia enterocolitica
Entry authors: Evdokimov AG, Waugh DS, Routzahn K, Tropea J, Cherry S

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase YopH Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.54 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15273 (Residues: 163-468; Coverage: 65%)
Gene names: yop51, yopH
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P212121
Unit cell:
a: 55.456Å b: 49.331Å c: 97.537Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 not available 0.19
Expression system: Escherichia coli BL21(DE3)

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