1lws Summary

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Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence

The structure was published by Moure, C.M., Gimble, F.S., and Quiocho, F.A., in 2002 in a paper entitled "Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.5 Å and deposited in 2002.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely PI-SceI DNA recognition region top strand, PI-SceI DNA recognition region bottom strand, and ENDONUCLEASE PI-SCEI.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A ENDONUCLEASE PI-SCEI P17255 (284-737) (VATA_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 454 94%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P17255 (284 - 737) ENDONUCLEASE PI-SCEI Saccharomyces cerevisiae

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A Intein (protein splicing domain)search, Intein endonucleasesearch Endonuclease - Pi-scei; Chain A, domain 1search, Homing endonucleasessearch Hom_end-associated Hintsearch

Chain ID Biological process (GO) Molecular function (GO)
A (P17255) protein splicingsearch intein-mediated protein splicingsearch endonuclease activitysearch DNA bindingsearch

Chain InterPro annotation
A Hint domain N-terminalsearch Intein DOD homing endonucleasesearch Intein splice sitesearch Inteinsearch Hom-end-associated Hintsearch Homing endonuclease PI-Scesearch Homing endonucleasesearch Hedgehog/Intein (Hint) domainsearch