1lvc Summary


Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 2' deoxy, 3' anthraniloyl ATP

The structure was published by Shen, Y., Lee, Y.-S., Soelaiman, S., et al., Grabarek, Z., Mrksich, M., and Tang, W.-J., in 2002 in a paper entitled "Physiological calcium concentrations regulate calmodulin binding and catalysis of adenylyl cyclase exotoxins" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.6 Å and deposited in 2002.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely calmodulin-sensitive adenylate cyclase and calmodulin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
B calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
C calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
D calmodulin P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 95%
E calmodulin P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 95%
F calmodulin P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 95%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (291 - 800) calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (1 - 149) calmodulin Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C (P40136) Adenylylcyclase toxin (the edema factor)search Adenylylcyclase toxin superfamilysearch PF03497: Anthrax toxin LF subunitsearch
D, E, F (P62158) Calmodulin-likesearch EF-handsearch PF00036: EF handsearch, PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
A, B, C (P40136) extracellular regionsearch calcium- and calmodulin-responsive adenylate cyclase activitysearch pathogenesissearch
D, E, F (P62158) calcium channel complexsearch spindle polesearch extracellular regionsearch nucleussearch nucleoplasmsearch cytoplasmsearch centrosomesearch cytosolsearch cytoskeletonsearch spindle microtubulesearch plasma membranesearch sarcomeresearch growth conesearch vesiclesearch neuron projectionsearch extracellular vesicular exosomesearch calcium ion bindingsearch protein bindingsearch adenylate cyclase bindingsearch protein kinase bindingsearch protein domain specific bindingsearch enzyme regulator activitysearch nitric-oxide synthase regulator activitysearch titin bindingsearch type 3 metabotropic glutamate receptor bindingsearch thioesterase bindingsearch N-terminal myristoylation domain bindingsearch phospholipase bindingsearch protein serine/threonine kinase activator activitysearch phosphatidylinositol 3-kinase bindingsearch ion channel bindingsearch metal ion bindingsearch protein N-terminus bindingsearch calcium-dependent protein bindingsearch nitric-oxide synthase bindingsearch protein phosphatase activator activitysearch response to amphetaminesearch regulation of heart ratesearch platelet degranulationsearch detection of calcium ionsearch carbohydrate metabolic processsearch glycogen catabolic processsearch glucose metabolic processsearch muscle contractionsearch signal transductionsearch epidermal growth factor receptor signaling pathwaysearch G-protein coupled receptor signaling pathwaysearch activation of adenylate cyclase activitysearch activation of phospholipase C activitysearch synaptic transmissionsearch blood coagulationsearch phototransduction, visible lightsearch fibroblast growth factor receptor signaling pathwaysearch positive regulation of peptidyl-threonine phosphorylationsearch negative regulation of peptidyl-threonine phosphorylationsearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch rhodopsin mediated signaling pathwaysearch calcium-mediated signalingsearch substantia nigra developmentsearch regulation of rhodopsin mediated signaling pathwaysearch platelet activationsearch positive regulation of cyclic nucleotide metabolic processsearch positive regulation of protein autophosphorylationsearch regulation of cytokinesissearch positive regulation of phosphoprotein phosphatase activitysearch positive regulation of protein dephosphorylationsearch Fc-epsilon receptor signaling pathwaysearch inositol phosphate metabolic processsearch small molecule metabolic processsearch innate immune responsesearch nitric oxide metabolic processsearch neurotrophin TRK receptor signaling pathwaysearch regulation of nitric-oxide synthase activitysearch positive regulation of nitric-oxide synthase activitysearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch response to corticosteronesearch response to calcium ionsearch regulation of cardiac muscle contractionsearch regulation of ryanodine-sensitive calcium-release channel activitysearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch membrane organizationsearch positive regulation of protein serine/threonine kinase activitysearch regulation of high voltage-gated calcium channel activitysearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch

Chain InterPro annotation
A, B, C Anthrax toxin, edema factor, centralsearch
D, E, F EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch