1lvc Summary

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Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin and 2' deoxy, 3' anthraniloyl ATP

The structure was published by Shen, Y., Lee, Y.-S., Soelaiman, S., et al., Grabarek, Z., Mrksich, M., and Tang, W.-J., in 2002 in a paper entitled "Physiological calcium concentrations regulate calmodulin binding and catalysis of adenylyl cyclase exotoxins" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.6 Å and deposited in 2002.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely calmodulin-sensitive adenylate cyclase and calmodulin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
B calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
C calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
D calmodulin P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 95%
E calmodulin P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 95%
F calmodulin P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 95%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (291 - 800) calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (1 - 149) calmodulin Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C (P40136) Adenylylcyclase toxin (the edema factor)search PF03497: Anthrax toxin LF subunitsearch
D, E, F (P62158) Calmodulin-likesearch EF-handsearch PF00036: EF handsearch, PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B, C (P40136) calcium- and calmodulin-responsive adenylate cyclase activitysearch pathogenesissearch extracellular regionsearch
D, E, F (P62158) calcium ion bindingsearch ion channel bindingsearch protein bindingsearch protein kinase bindingsearch phospholipase bindingsearch protein serine/threonine kinase activator activitysearch protein domain specific bindingsearch metal ion bindingsearch enzyme regulator activitysearch thioesterase bindingsearch type 3 metabotropic glutamate receptor bindingsearch nitric-oxide synthase regulator activitysearch protein N-terminus bindingsearch titin bindingsearch phosphatidylinositol 3-kinase bindingsearch adenylate cyclase bindingsearch calcium-dependent protein bindingsearch protein phosphatase activator activitysearch N-terminal myristoylation domain bindingsearch nitric-oxide synthase bindingsearch phototransduction, visible lightsearch inositol phosphate metabolic processsearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch detection of calcium ionsearch regulation of nitric-oxide synthase activitysearch positive regulation of nitric-oxide synthase activitysearch response to corticosteronesearch muscle contractionsearch neurotrophin TRK receptor signaling pathwaysearch glycogen catabolic processsearch regulation of cardiac muscle contractionsearch G-protein coupled receptor signaling pathwaysearch response to calcium ionsearch positive regulation of peptidyl-threonine phosphorylationsearch membrane organizationsearch synaptic transmissionsearch regulation of high voltage-gated calcium channel activitysearch activation of adenylate cyclase activitysearch positive regulation of phosphoprotein phosphatase activitysearch Fc-epsilon receptor signaling pathwaysearch positive regulation of protein dephosphorylationsearch signal transductionsearch rhodopsin mediated signaling pathwaysearch regulation of cytokinesissearch platelet degranulationsearch epidermal growth factor receptor signaling pathwaysearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch glucose metabolic processsearch negative regulation of peptidyl-threonine phosphorylationsearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch regulation of rhodopsin mediated signaling pathwaysearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch regulation of heart ratesearch response to amphetaminesearch nitric oxide metabolic processsearch innate immune responsesearch positive regulation of protein autophosphorylationsearch fibroblast growth factor receptor signaling pathwaysearch positive regulation of cyclic nucleotide metabolic processsearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch blood coagulationsearch platelet activationsearch activation of phospholipase C activitysearch calcium-mediated signalingsearch regulation of ryanodine-sensitive calcium-release channel activitysearch substantia nigra developmentsearch positive regulation of protein serine/threonine kinase activitysearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch carbohydrate metabolic processsearch small molecule metabolic processsearch nucleoplasmsearch cytosolsearch nucleussearch spindle polesearch centrosomesearch plasma membranesearch sarcomeresearch growth conesearch cytoplasmsearch spindle microtubulesearch extracellular vesicular exosomesearch neuron projectionsearch calcium channel complexsearch vesiclesearch cytoskeletonsearch spindlesearch extracellular regionsearch

Chain InterPro annotation
A, B, C Anthrax toxin, edema factor, centralsearch
D, E, F EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch