› 1lpc

X-ray diffraction
1.7Å resolution

HIGH RESOLUTION STRUCTURE OF RECOMBINANT DIANTHIN ANTIVIRAL PROTEIN-POTENT ANTI-HIV AGENT (COMPLEX WITH CYCLIC AMP)

Released:
DOI: 10.2210/pdb1lpc/pdb
PDB entry 1lpc coloured by chain, front view.
PDB entry 1lpc coloured by chain, side view.
PDB entry 1lpc coloured by chain, top view.
Source organism: Dianthus caryophyllus
Primary publication:
High resolution X-ray structure and potent anti-HIV activity of recombinant dianthin antiviral protein.
Kurinov IV, Rajamohan F, Uckun FM
Arzneimittelforschung 54 692-702 (2004)
PMID: 15553110

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein dianthin-30 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.6 KDa
Source organism: Dianthus caryophyllus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24476 (Residues: 24-277; Coverage: 94%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CMP 1 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 37.122Å b: 78.498Å c: 42.715Å
α: 90° β: 105.228° γ: 90°
R-values:
R R work R free
0.195 0.19 0.24
Expression system: Escherichia coli

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Citations

1 review citation

Biological activities of ribosome-inactivating proteins and their possible applications as antimicrobial, anticancer, and anti-pest agents and in neuroscience research.
Akkouh et al. (2015)

2 mentions without citation

Dianthin and Its Potential in Targeted Tumor Therapies.
Fuchs H. (2019)
1 more