› 1lnx

X-ray diffraction
2.05Å resolution

Crystal structure of the P.aerophilum SmAP1 heptamer in a new crystal form (C2221)

Released:
DOI: 10.2210/pdb1lnx/pdb
PDB entry 1lnx coloured by chain, front view.
PDB entry 1lnx coloured by chain, side view.
PDB entry 1lnx coloured by chain, top view.
Source organism: Pyrobaculum aerophilum
Primary publication:
The oligomerization and ligand-binding properties of Sm-like archaeal proteins (SmAPs).
Mura C, Kozhukhovsky A, Gingery M, Phillips M, Eisenberg D
Protein Sci 12 832-47 (2003)
PMID: 12649441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo heptamer (preferred)
homo tetradecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-187432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative snRNP Sm-like protein Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 81 amino acids
Theoretical weight: 8.94 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZYG5 (Residues: 1-80; Coverage: 100%)
Gene name: PAE0790
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

3 bound ligands:
Ligand URI 7 x URI
Ligand GOL 10 x GOL
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 91.829Å b: 113.764Å c: 126.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Hfq structure, function and ligand binding.
Brennan et al. (2007)
6 more

2 mentions without citation

Library of disordered patterns in 3D protein structures.
Lobanov et al. (2010)
1 more