› 1lmb

X-ray diffraction
1.8Å resolution

REFINED 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR-OPERATOR COMPLEX

Released:
DOI: 10.2210/pdb1lmb/pdb
PDB entry 1lmb coloured by chain, front view.
PDB entry 1lmb coloured by chain, side view.
PDB entry 1lmb coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Refined 1.8 A crystal structure of the lambda repressor-operator complex.
Beamer LJ, Pabo CO
J Mol Biol 227 177-96 (1992)
PMID: 1387915

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114142 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Repressor protein cI Chains: 3, 4
Molecule details ›
Chains: 3, 4
Length: 92 amino acids
Theoretical weight: 10.14 KDa
Source organism: Escherichia virus Lambda
UniProt:
  • Canonical: P03034 (Residues: 2-93; Coverage: 39%)
Gene names: cI, lambdap88
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*AP*TP*AP*CP*CP*AP*CP*TP*GP*GP*CP*GP*GP*TP*GP*A P*TP*AP*T)-3') Chain: 1
Molecule details ›
Chain: 1
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
DNA (5'-D(*TP*AP*TP*AP*TP*CP*AP*CP*CP*GP*CP*CP*AP*GP*TP*GP*G P*TP*AP*T)-3') Chain: 2
Molecule details ›
Chain: 2
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 37.22Å b: 68.72Å c: 57.03Å
α: 90° β: 92.2° γ: 90°
R-values:
R R work R free
0.189 0.189 not available
Expression system: Not provided

Quick links

Citations

11 review citations

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
10 more

100 mentions without citation

An overview of recent advances in duplex DNA recognition by small molecules.
Bhaduri et al. (2018)
99 more