› 1lln

X-ray diffraction
1.6Å resolution

1.6A CRYSTAL STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN-III (PAP-III) WITH METHYLATED LYSINES

Released:
DOI: 10.2210/pdb1lln/pdb
PDB entry 1lln coloured by chain, front view.
PDB entry 1lln coloured by chain, side view.
PDB entry 1lln coloured by chain, top view.
Source organism: Phytolacca americana
Primary publication:
High resolution X-ray structure of potent anti-HIV pokeweed antiviral protein-III.
Kurinov IV, Uckun FM
Biochem Pharmacol 65 1709-17 (2003)
PMID: 12754107

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral protein II/III Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.68 KDa
Source organism: Phytolacca americana
UniProt:
  • Canonical: Q40772 (Residues: 26-287; Coverage: 92%)
Gene names: PAP2, PAPII
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 14 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3121
Unit cell:
a: 80.47Å b: 80.47Å c: 76.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.245

Quick links

Citations

4 review citations

Naturally derived anti-HIV agents.
Asres et al. (2005)
3 more

1 mention without citation

New surface contacts formed upon reductive lysine methylation: improving the probability of protein crystallization.
Sledz et al. (2010)