› 1lfu

Solution NMR

NMR Solution Structure of the Extended PBX Homeodomain Bound to DNA

Released:
DOI: 10.2210/pdb1lfu/pdb
PDB entry 1lfu coloured by chain, ensemble of 20 models, front view.
PDB entry 1lfu coloured by chain, ensemble of 20 models, side view.
PDB entry 1lfu coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Primary publication:
Lock and key binding of the HOX YPWM peptide to the PBX homeodomain.
Sprules T, Green N, Featherstone M, Gehring K
J Biol Chem 278 1053-8 (2003)
PMID: 12409300

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114138 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Pre-B-cell leukemia transcription factor 1 Chain: P
Molecule details ›
Chain: P
Length: 82 amino acids
Theoretical weight: 9.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P41778 (Residues: 233-313; Coverage: 19%)
Gene names: Pbx-1, Pbx1
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
5'-D(*GP*CP*GP*CP*AP*TP*GP*AP*TP*TP*GP*CP*CP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*GP*GP*GP*CP*AP*AP*TP*CP*AP*TP*GP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.31 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: dynamical annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Hox genes and their candidate downstream targets in the developing central nervous system.
Akin et al. (2005)
2 more

3 mentions without citation

Quantitative evaluation of protein-DNA interactions using an optimized knowledge-based potential.
Liu et al. (2005)
2 more