› 1lf9

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE

Released:
DOI: 10.2210/pdb1lf9/pdb
PDB entry 1lf9 coloured by chain, front view.
PDB entry 1lf9 coloured by chain, side view.
PDB entry 1lf9 coloured by chain, top view.
Source organism: Thermoanaerobacterium thermosaccharolyticum
Primary publication:
Crystal structure and evolution of a prokaryotic glucoamylase.
Aleshin AE, Feng PH, Honzatko RB, Reilly PJ
J Mol Biol 327 61-73 (2003)
PMID: 12614608

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucoamylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 684 amino acids
Theoretical weight: 76.59 KDa
Source organism: Thermoanaerobacterium thermosaccharolyticum
UniProt:
  • Canonical: O85672 (Residues: 18-141, 142-695; Coverage: 100%)
Gene name: ga1
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, AC1
Ligand GLC 4 x GLC
Ligand AC1 2 x AC1
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 80.491Å b: 102.931Å c: 164.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.227

Quick links

Citations

5 review citations

Microbial glucoamylases: characteristics and applications.
Kumar et al. (2009)
4 more

2 mentions without citation

Functional classification of protein structures by local structure matching in graph representation.
Mills et al. (2018)
1 more