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Crystal Structure of H-2Kbm3 bound to dEV8

X-ray diffraction
2.15Å resolution
PDB entry 1lek coloured by chain, front view.
PDB entry 1lek coloured by chain, side view.
PDB entry 1lek coloured by chain, top view.
Source organism: Mus musculus
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 18919.16 Å2
Buried surface area: 5468.87 Å2
Dissociation area: 869.66 Å2
Dissociation energy (ΔGdiss): 5.51 kcal/mol
Dissociation entropy (TΔSdiss): 7.39 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135059

Macromolecules

H-2 class I histocompatibility antigen, K-B alpha chain
Chain: A
Length: 274 amino acids
Theoretical weight: 31.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Pfam:
InterPro:
CATH:
SCOP:
H-2 class I histocompatibility antigen, K-B alpha chain in PDB entry 1lek, assembly 1, front view.
H-2 class I histocompatibility antigen, K-B alpha chain in PDB entry 1lek, assembly 1, side view.
H-2 class I histocompatibility antigen, K-B alpha chain in PDB entry 1lek, assembly 1, top view.
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Beta-2-microglobulin
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Pfam: Immunoglobulin C1-set domain
InterPro:
CATH: Immunoglobulins
SCOP: C1 set domains (antibody constant domain-like)
Beta-2-microglobulin in PDB entry 1lek, assembly 1, front view.
Beta-2-microglobulin in PDB entry 1lek, assembly 1, side view.
Beta-2-microglobulin in PDB entry 1lek, assembly 1, top view.
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Cytochrome c oxidase subunit NDUFA4
Chain: P
Length: 8 amino acids
Theoretical weight: 1.06 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q62425 (Residues: 61-68; Coverage: 10%)
Gene name: Ndufa4
Cytochrome c oxidase subunit NDUFA4 in PDB entry 1lek, assembly 1, front view.
Cytochrome c oxidase subunit NDUFA4 in PDB entry 1lek, assembly 1, side view.
Cytochrome c oxidase subunit NDUFA4 in PDB entry 1lek, assembly 1, top view.
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