› 1lei

X-ray diffraction
2.7Å resolution

The kB DNA sequence from the HLV-LTR functions as an allosteric regulator of HIV transcription

Released:
DOI: 10.2210/pdb1lei/pdb
PDB entry 1lei coloured by chain, front view.
PDB entry 1lei coloured by chain, side view.
PDB entry 1lei coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The kappa B DNA sequence from the HIV long terminal repeat functions as an allosteric regulator of HIV transcription.
Chen-Park FE, Huang DB, Noro B, Thanos D, Ghosh G
J Biol Chem 277 24701-8 (2002)
PMID: 11970949

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114134 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor p65 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.12 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04207 (Residues: 20-291; Coverage: 50%)
Gene names: Nfkb3, Rela
Sequence domains:
Structure domains:
Nuclear factor NF-kappa-B p50 subunit Chain: B
Molecule details ›
Chain: B
Length: 313 amino acids
Theoretical weight: 35.06 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25799 (Residues: 39-350; Coverage: 32%)
Gene name: Nfkb1
Sequence domains:
Structure domains:
5'-D(*CP*TP*CP*AP*GP*GP*GP*AP*AP*AP*GP*TP*AP*CP*AP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.27 KDa
5'-D(*TP*CP*TP*GP*5ITP*AP*CP*5ITP*5ITP*5ITP*CP*CP*CP*TP*GP*AP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.59 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5IU 4 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C222
Unit cell:
a: 137.131Å b: 179.697Å c: 97.068Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Transcriptional control of the inflammatory response.
Medzhitov et al. (2009)
14 more

4 mentions without citation

An atomic model of the interferon-beta enhanceosome.
Panne et al. (2007)
3 more