› 1lcv

X-ray diffraction
2.3Å resolution

streptavidin-norbiotin complex

Released:
DOI: 10.2210/pdb1lcv/pdb
PDB entry 1lcv coloured by chain, front view.
PDB entry 1lcv coloured by chain, side view.
PDB entry 1lcv coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Ligand exchange between proteins. Exchange of biotin and biotin derivatives between avidin and streptavidin.
Pazy Y, Kulik T, Bayer EA, Wilchek M, Livnah O
J Biol Chem 277 30892-900 (2002)
PMID: 12055191

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 12.76 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 39-159; Coverage: 76%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand SNR 2 x SNR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I222
Unit cell:
a: 47.29Å b: 95.32Å c: 105.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.233
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Essentials of biorecognition: the (strept)avidin-biotin system as a model for protein-protein and protein-ligand interaction.
Wilchek et al. (2006)
2 more

2 mentions without citation

Dynamics of the streptavidin-biotin complex in solution and in its crystal lattice: distinct behavior revealed by molecular simulations.
Cerutti et al. (2009)
1 more