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EBI PDBe PDBeView

PDBe Entry: 1lb2 view

Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA
Summary
Header GENE REGULATION/DNAsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 3.1 Å, R-factor: 21.1%, Free R-factor: 24.4%, Spacegroup: P 62 2 2
Released 06/09/2002, deposition: 01/04/2002, last revision: 24/02/2009
Authors Benoff, B.search; Yang, H.search; Lawson, C.L.search; Parkinson, G.search; Liu, J.search; Blatter, E.search; Ebright, Y.W.search; Berman, H.M.search; Ebright, R.H.search
Primary citation Structural basis of transcription activation: the CAP-alpha CTD-DNA complex.
SCIENCEsearch vol:297, pag:1562-1566 (2002) [PubMed ID 12202833 ]search
Keywords PROTEIN-DNA COMPLEXsearch, GENE-REGULATORYsearch, GENE REGULATION/DNA COMPLEXsearch
EC 2.7.7.6 ExPASy BRENDA search (B E)
Organism Escherichia coli 562search(A B E)
UniProt Catabolite gene activator (cAMP receptor protein) (cAMP regulatory protein) P0ACJ8search (A)
DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) (RNAP subunit alpha) (Transcriptase subunit alpha) (RNA polymerase subunit alpha) P0A7Z4search (B E)
Solvent A, B, E, J, K
Polymers
Id Name Type UniProt Residues Observed
K 5'-D(*CP*TP*TP*TP*TP*TP*TP*CP*CP*TP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3' DNA
20 100%
J 5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*AP*G)-3' DNA
24 100%
A CATABOLITE GENE ACTIVATOR PROTEIN Protein P0ACJ8 (CRP_ECOLI)search
 209 96%
B, E DNA-directed RNA polymerase alpha chain Protein P0A7Z4 (RPOA_ECOLI)search
 84 85%
Heterogens
Id Name Ligands
A ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE CMP search
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