PDBe Entry: 1l9u

THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION

Summary

Header

TRANSCRIPTION

Method

X-RAY DIFFRACTION

Experiment

Resolution: 4.0 Å, Spacegroup: P 1 21 1

Released

22/05/2002, deposition: 26/03/2002, last revision: 24/02/2009

Authors

Murakami, K.S.

; Masuda, S.

; Darst, S.A.

Primary citation

Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution.
SCIENCE

vol:296, pag:1280-1284 (2002) [PubMed ID 12016306 ]

Keywords

helix-turn-helix

, coiled-coil

, TRANSCRIPTION

(A B C D E J K L M N)

Organism

Thermus aquaticus 271

(A B J K C L D M E N H Q)

UniProt

DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) (RNAP subunit alpha) (Transcriptase subunit alpha) (RNA polymerase subunit alpha) Q9KWU8

(A B J K)
DNA-directed RNA polymerase subunit beta (EC 2.7.7.6) (RNAP subunit beta) (Transcriptase subunit beta) (RNA polymerase subunit beta) Q9KWU7

(C L)
DNA-directed RNA polymerase subunit beta' (EC 2.7.7.6) (RNAP subunit beta') (Transcriptase subunit beta') (RNA polymerase subunit beta') Q9KWU6

(D M)
DNA-directed RNA polymerase subunit omega (EC 2.7.7.6) (RNAP omega subunit) (Transcriptase subunit omega) (RNA polymerase omega subunit) Q9EVV4

(E N)
RNA polymerase sigma factor,Sigma factor SigA Q9EZJ8

(H Q)

Polymers

Id	Name	Type	UniProt	Residues	Observed
A, B, J, K	RNA POLYMERASE, ALPHA SUBUNIT	Protein	Q9KWU8 (RPOA_THEAQ)	314	71%
C, L	RNA POLYMERASE, BETA SUBUNIT	Protein	Q9KWU7 (RPOB_THEAQ)	1118	96%
D, M	RNA POLYMERASE, BETA-PRIME SUBUNIT	Protein	Q9KWU6 (RPOC_THEAQ)	1524	77%
E, N	RNA POLYMERASE, OMEGA SUBUNIT	Protein	Q9EVV4 (RPOZ_THEAQ)	99	92%
H, Q	SIGMA FACTOR SIGA	Protein	Q9EZJ8 (Q9EZJ8_THEAQ)	332	96%

Heterogens