› 1l2l

X-ray diffraction
2Å resolution

Crystal structure of ADP-dependent glucokinase from a Pyrococcus Horikoshii

Released:
DOI: 10.2210/pdb1l2l/pdb
PDB entry 1l2l coloured by chain, front view.
PDB entry 1l2l coloured by chain, side view.
PDB entry 1l2l coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase.
Tsuge H, Sakuraba H, Kobe T, Kujime A, Katunuma N, Ohshima T
Protein Sci 11 2456-63 (2002)
PMID: 12237466

Function and Biology Details

Reaction catalysed: 
ADP + D-glucosamine = AMP + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129710 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-dependent glucose/glucosamine kinase Chain: A
Molecule details ›
Chain: A
Length: 457 amino acids
Theoretical weight: 52.17 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O58328 (Residues: 1-457; Coverage: 100%)
Gene names: PH0589, glkA
Sequence domains: ADP-specific Phosphofructokinase/Glucokinase conserved region
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 64.76Å b: 74.73Å c: 99.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.277
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
5 more

7 mentions without citation

Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase.
Tsuge et al. (2002)
6 more