1kp2 Summary


Crystal Structure of E. coli Argininosuccinate Synthetase in Complex with ATP

The structure was published by Lemke, C.T. and Howell, P.L., in 2002 in a paper entitled "Substrate Induced Conformational Changes in Argininosuccinate Synthetase" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 2001.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of argininosuccinate synthetase. This molecule has the UniProt identifier P0A6E4 (ASSY_ECOLI)search. The sample contained 455 residues which is 100% of the natural sequence. Out of 455 residues 428 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A argininosuccinate synthetase P0A6E4 (2-447) (ASSY_ECOLI)search Escherichia coli K-12search 100% 455 94%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P0A6E4 (2 - 447) argininosuccinate synthetase Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P0A6E4) N-type ATP pyrophosphatasessearch, Argininosuccinate synthetase, C-terminal domainsearch HUPssearch, Argininosuccinate synthetase, chain A, domain 2search, Helix Hairpinssearch PF00764: Arginosuccinate synthasesearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A (P0A6E4) argininosuccinate synthase activitysearch nucleotide bindingsearch protein bindingsearch ATP bindingsearch ligase activitysearch protein homodimerization activitysearch cytoplasmsearch arginine biosynthetic processsearch argininosuccinate metabolic processsearch urea cyclesearch cellular amino acid biosynthetic processsearch

Chain InterPro annotation
A Argininosuccinate synthasesearch Rossmann-like alpha/beta/alpha sandwich foldsearch Argininosuccinate synthase, conserved sitesearch Argininosuccinate synthase, type 2 subfamilysearch Argininosuccinate synthetase mutimerisation domain, C-terminal tailsearch Argininosuccinate synthetase, catalytic/multimerisation domain bodysearch