› 1kkq

X-ray diffraction
3Å resolution

Crystal structure of the human PPAR-alpha ligand-binding domain in complex with an antagonist GW6471 and a SMRT corepressor motif

Released:
DOI: 10.2210/pdb1kkq/pdb
PDB entry 1kkq coloured by chain, front view.
PDB entry 1kkq coloured by chain, side view.
PDB entry 1kkq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for antagonist-mediated recruitment of nuclear co-repressors by PPARalpha.
Xu HE, Stanley TB, Montana VG, Lambert MH, Shearer BG, Cobb JE, McKee DD, Galardi CM, Plunket KD, Nolte RT, Parks DJ, Moore JT, Kliewer SA, Willson TM, Stimmel JB
Nature 415 813-7 (2002)
PMID: 11845213

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170556 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peroxisome proliferator-activated receptor alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 269 amino acids
Theoretical weight: 30.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07869 (Residues: 200-468; Coverage: 58%)
Gene names: NR1C1, PPAR, PPARA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor corepressor 2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 19 amino acids
Theoretical weight: 2.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y618 (Residues: 2336-2354; Coverage: 1%)
Gene names: CTG26, NCOR2

Ligands and Environments

1 bound ligand:
Ligand 471 4 x 471
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 103.409Å b: 112.773Å c: 123.954Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.258 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

71 review citations

Estrogen receptors: how do they signal and what are their targets.
Heldring et al. (2007)
70 more

30 mentions without citation

Androgen receptor: structure, role in prostate cancer and drug discovery.
Tan et al. (2015)
29 more