1khl Summary



The structure was published by Le Du, M.H., Lamoure, C., Muller, B.H., et al., Lajeunesse, E., Menez, A., and Boulain, J.C., in 2002 in a paper entitled "Artificial evolution of an enzyme active site: structural studies of three highly active mutants of Escherichia coli alkaline phosphatase." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.5 Å and deposited in 2001.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of Alkaline Phosphatase.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Alkaline Phosphatase P00634 (23-471) (PPB_ECOLI)search Escherichia coli K-12search 99% 449 98%
B Alkaline Phosphatase P00634 (23-471) (PPB_ECOLI)search Escherichia coli K-12search 99% 449 98%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P00634 (23 - 471) Alkaline Phosphatase Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (P00634) Alkaline phosphatasesearch Alkaline Phosphatase, subunit Asearch PF00245: Alkaline phosphatasesearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P00634) phosphatase activitysearch oxidoreductase activity, acting on phosphorus or arsenic in donorssearch alkaline phosphatase activitysearch zinc ion bindingsearch protein bindingsearch catalytic activitysearch magnesium ion bindingsearch hydrolase activitysearch hydrogenase (acceptor) activitysearch metal ion bindingsearch metabolic processsearch dephosphorylationsearch oxidation-reduction processsearch periplasmic spacesearch outer membrane-bounded periplasmic spacesearch

Chain InterPro annotation
A, B Alkaline phosphatasesearch Alkaline phosphatase-like, alpha/beta/alphasearch Alkaline-phosphatase-like, core domainsearch Alkaline phosphatase, active sitesearch