1khe Summary


PEPCK complex with nonhydrolyzable GTP analog, MAD data

The structure was published by Dunten, P., Belunis, C., Crowther, R., et al., Swain, A., Weber, D., and Wertheimer, S.J., in 2002 in a paper entitled "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.4 Å and deposited in 2001.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Phosphoenolpyruvate Carboxykinase, cytosolic (GTP). This molecule has the UniProt identifier P35558 (PCKGC_HUMAN)search. The sample contained 625 residues which is 100% of the natural sequence. Out of 625 residues 602 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Phosphoenolpyruvate Carboxykinase, cytosolic (GTP) P35558 (1-622) (PCKGC_HUMAN)search Homo sapienssearch 100% 625 96%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P35558 (1 - 622) Phosphoenolpyruvate Carboxykinase, cytosolic (GTP) Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P35558) PEP carboxykinase C-terminal domainsearch, PEP carboxykinase N-terminal domainsearch Phosphoenolpyruvate Carboxykinase, domain 1search, Phosphoenolpyruvate Carboxykinase, domain 2search, Phosphoenolpyruvate Carboxykinase; domain 3search PF00821: Phosphoenolpyruvate carboxykinasesearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A (P35558) phosphoenolpyruvate carboxykinase (GTP) activitysearch carboxylic acid bindingsearch GTP bindingsearch purine nucleotide bindingsearch phosphoenolpyruvate carboxykinase activitysearch nucleotide bindingsearch lyase activitysearch magnesium ion bindingsearch manganese ion bindingsearch metal ion bindingsearch GDP bindingsearch carboxy-lyase activitysearch extracellular vesicular exosomesearch cytoplasmsearch cytosolsearch glucose metabolic processsearch drug metabolic processsearch gluconeogenesissearch carbohydrate metabolic processsearch glycerol biosynthetic process from pyruvatesearch glucose homeostasissearch lipid metabolic processsearch response to activitysearch response to insulinsearch oxaloacetate metabolic processsearch small molecule metabolic processsearch internal protein amino acid acetylationsearch

Chain InterPro annotation
A Phosphoenolpyruvate carboxykinase, GTP-utilisingsearch Phosphoenolpyruvate carboxykinase, N-terminalsearch Phosphoenolpyruvate carboxykinase, C-terminalsearch Phosphoenolpyruvate carboxykinase, GTP-utilising, conserved sitesearch