1kfx Summary

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Crystal Structure of Human m-Calpain Form I

The structure was published by Strobl, S., Fernandez-Catalan, C., Braun, M., et al., Bartunik, H., Suzuki, K., and Bode, W., in 2000 in a paper entitled "The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.15 Å and deposited in 2001.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely M-CALPAIN LARGE SUBUNIT and M-CALPAIN SMALL SUBUNIT.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
L M-CALPAIN LARGE SUBUNIT P17655 (2-700) (CAN2_HUMAN)search Homo sapienssearch 100% 699 91%
S M-CALPAIN SMALL SUBUNIT P04632 (85-268) (CPNS1_HUMAN)search Homo sapienssearch < 90% 184 95%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P17655 (2 - 700) M-CALPAIN LARGE SUBUNIT Homo sapiens
P04632 (85 - 268) M-CALPAIN SMALL SUBUNIT Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
L (P17655) Penta-EF-hand proteinssearch, Calpain large subunit, middle domain (domain III)search, Calpain large subunit, catalytic domain (domain II)search Cysteine proteinasessearch, Jelly Rollssearch, EF-handsearch PF00648: Calpain family cysteine proteasesearch, PF01067: Calpain large subunit, domain IIIsearch, PF13833: EF-hand domain pairsearch
S Penta-EF-hand proteinssearch EF-handsearch EF-hand domainsearch, EF-hand domain pairsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
L (P17655) calcium-dependent cysteine-type endopeptidase activitysearch protein bindingsearch cysteine-type peptidase activitysearch cytoskeletal protein bindingsearch hydrolase activitysearch metal ion bindingsearch peptidase activitysearch calcium ion bindingsearch protein heterodimerization activitysearch proteolysissearch cellular response to amino acid stimulussearch myoblast fusionsearch blastocyst developmentsearch regulation of cytoskeleton organizationsearch response to hypoxiasearch protein autoprocessingsearch proteolysis involved in cellular protein catabolic processsearch cytoplasmsearch cytosolsearch pseudopodiumsearch nucleussearch chromatinsearch cortical actin cytoskeletonsearch membrane raftsearch dendritesearch endoplasmic reticulumsearch Golgi apparatussearch plasma membranesearch extracellular vesicular exosomesearch perinuclear endoplasmic reticulumsearch intracellularsearch focal adhesionsearch membranesearch
S (P04632) calcium ion bindingsearch proteolysissearch

Chain InterPro annotation
L Cysteine peptidase, cysteine active sitesearch Peptidase C2, calpain, catalytic domainsearch EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch Peptidase C2, calpain, large subunit, domain IIIsearch Peptidase C2, calpain, domain IIIsearch Peptidase C2, calpain familysearch Calpain-2search
S EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch Calpain small subunit 1search