› 1kfu

X-ray diffraction
2.5Å resolution

Crystal Structure of Human m-Calpain Form II

Released:
DOI: 10.2210/pdb1kfu/pdb
PDB entry 1kfu coloured by chain, front view.
PDB entry 1kfu coloured by chain, side view.
PDB entry 1kfu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium.
Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K, Irie A, Sorimachi H, Bourenkow G, Bartunik H, Suzuki K, Bode W
Proc Natl Acad Sci U S A 97 588-92 (2000)
PMID: 10639123

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138096 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calpain-2 catalytic subunit Chain: L
Molecule details ›
Chain: L
Length: 699 amino acids
Theoretical weight: 79.97 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P17655 (Residues: 2-700; Coverage: 100%)
Gene names: CANPL2, CAPN2
Sequence domains:
Structure domains:
Calpain small subunit 1 Chain: S
Molecule details ›
Chain: S
Length: 184 amino acids
Theoretical weight: 21.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04632 (Residues: 85-268; Coverage: 69%)
Gene names: CAPN4, CAPNS, CAPNS1
Structure domains: EF-hand

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 51.88Å b: 169.84Å c: 64.44Å
α: 90° β: 95.12° γ: 90°
R-values:
R R work R free
0.221 0.221 0.276
Expression system: Spodoptera frugiperda

Quick links

Citations

58 review citations

The calpain system.
Goll et al. (2003)
57 more

21 mentions without citation

Structural similarity enhances interaction propensity of proteins.
Lukatsky et al. (2007)
20 more