› 1kao

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF THE SMALL G PROTEIN RAP2A WITH GDP

Released:
DOI: 10.2210/pdb1kao/pdb
PDB entry 1kao coloured by chain, front view.
PDB entry 1kao coloured by chain, side view.
PDB entry 1kao coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the small G protein Rap2A in complex with its substrate GTP, with GDP and with GTPgammaS.
Cherfils J, Ménétrey J, Le Bras G, Janoueix-Lerosey I, de Gunzburg J, Garel JR, Auzat I
EMBO J 16 5582-91 (1997)
PMID: 9312017

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rap-2a Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 18.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10114 (Residues: 1-167; Coverage: 91%)
Gene name: RAP2A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 36.826Å b: 35.315Å c: 58.356Å
α: 90° β: 106.67° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
7 more

10 mentions without citation

The structural GDP/GTP cycle of human Arf6.
Pasqualato et al. (2001)
9 more