› 1kan

X-ray diffraction
3Å resolution

MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1kan/pdb
PDB entry 1kan coloured by chain, front view.
PDB entry 1kan coloured by chain, side view.
PDB entry 1kan coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Molecular structure of kanamycin nucleotidyltransferase determined to 3.0-A resolution.
Sakon J, Liao HH, Kanikula AM, Benning MM, Rayment I, Holden HM
Biochemistry 32 11977-84 (1993)
PMID: 8218273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138345 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kanamycin nucleotidyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.9 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
UniProt:
  • Canonical: P05057 (Residues: 1-253; Coverage: 100%)
Gene names: kan, knt
Sequence domains: KNTase C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 78.9Å b: 78.9Å c: 219.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 not available not available
Expression system: Not provided

Quick links

Citations

14 review citations

Aminoglycosides: activity and resistance.
Mingeot-Leclercq et al. (1999)
13 more

7 mentions without citation

The ubiquitination machinery of the ubiquitin system.
Callis J. (2014)
6 more