› 1k9a

X-ray diffraction
2.5Å resolution

Crystal structure analysis of full-length carboxyl-terminal Src kinase at 2.5 A resolution

Released:
DOI: 10.2210/pdb1k9a/pdb
PDB entry 1k9a coloured by chain, front view.
PDB entry 1k9a coloured by chain, side view.
PDB entry 1k9a coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of the carboxyl-terminal Src kinase, Csk.
Ogawa A, Takayama Y, Sakai H, Chong KT, Takeuchi S, Nakagawa A, Nada S, Okada M, Tsukihara T
J Biol Chem 277 14351-4 (2002)
PMID: 11884384

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase CSK Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 450 amino acids
Theoretical weight: 50.81 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32577 (Residues: 1-450; Coverage: 100%)
Gene name: Csk
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 115Å b: 162.6Å c: 232.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.288
Expression system: Spodoptera frugiperda

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Citations

23 review citations

Regulation of protein kinases; controlling activity through activation segment conformation.
Nolen et al. (2004)
22 more

33 mentions without citation

T-cell signaling regulated by the Tec family kinase, Itk.
Andreotti et al. (2010)
32 more