1k30

X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of Squash (Cucurbita moschata) glycerol-3-phosphate (1)-acyltransferase

Released:

Function and Biology Details

Reactions catalysed:
Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate
Acyl-[acyl-carrier-protein] + sn-glycerol 3-phosphate = [acyl-carrier-protein] + 1-acyl-sn-glycerol 3-phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-145284 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol-3-phosphate acyltransferase ATS12, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 40.97 KDa
Source organism: Cucurbita moschata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10349 (Residues: 98-462; Coverage: 79%)
Gene names: AT2, ATS1;2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 61.134Å b: 65.098Å c: 103.298Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.224
Expression system: Escherichia coli BL21(DE3)