› 1k0i

X-ray diffraction
1.8Å resolution

Pseudomonas aeruginosa phbh R220Q in complex with 100mM PHB

Released:
DOI: 10.2210/pdb1k0i/pdb
PDB entry 1k0i coloured by chain, front view.
PDB entry 1k0i coloured by chain, side view.
PDB entry 1k0i coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Protein and ligand dynamics in 4-hydroxybenzoate hydroxylase.
Wang J, Ortiz-Maldonado M, Entsch B, Massey V, Ballou D, Gatti DL
Proc Natl Acad Sci U S A 99 608-13 (2002)
PMID: 11805318

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p-hydroxybenzoate hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.35 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P20586 (Residues: 1-394; Coverage: 100%)
Gene names: PA0247, pobA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand SO4 8 x SO4
Ligand SO3 1 x SO3
Ligand PHB 2 x PHB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 70.678Å b: 138.15Å c: 92.509Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.24
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts.
van Berkel et al. (2006)
12 more

4 mentions without citation

Protein and ligand dynamics in 4-hydroxybenzoate hydroxylase.
Wang et al. (2002)
3 more