› 1k0g

X-ray diffraction
2.05Å resolution

THE CRYSTAL STRUCTURE OF AMINODEOXYCHORISMATE SYNTHASE FROM PHOSPHATE GROWN CRYSTALS

Released:
DOI: 10.2210/pdb1k0g/pdb
PDB entry 1k0g coloured by chain, front view.
PDB entry 1k0g coloured by chain, side view.
PDB entry 1k0g coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Escherichia coli aminodeoxychorismate synthase: architectural conservation and diversity in chorismate-utilizing enzymes.
Parsons JF, Jensen PY, Pachikara AS, Howard AJ, Eisenstein E, Ladner JE
Biochemistry 41 2198-208 (2002)
PMID: 11841211

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-138333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxychorismate synthase component 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 453 amino acids
Theoretical weight: 51.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P05041 (Residues: 1-453; Coverage: 100%)
Gene names: JW1801, b1812, pabB
Sequence domains:
Structure domains: Anthranilate synthase

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand TRP 2 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 102.01Å b: 109.71Å c: 134.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 not available 0.238
Expression system: Escherichia coli

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Citations

5 review citations

Biosynthesis of enediyne antitumor antibiotics.
Van Lanen et al. (2008)
4 more

3 mentions without citation

The structure of MbtI from Mycobacterium tuberculosis, the first enzyme in the biosynthesis of the siderophore mycobactin, reveals it to be a salicylate synthase.
Harrison et al. (2006)
2 more