› 1jz6

X-ray diffraction
2.1Å resolution

E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH GALACTO-TETRAZOLE

Released:
DOI: 10.2210/pdb1jz6/pdb
PDB entry 1jz6 coloured by chain, front view.
PDB entry 1jz6 coloured by chain, side view.
PDB entry 1jz6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A structural view of the action of Escherichia coli (lacZ) beta-galactosidase.
Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW
Biochemistry 40 14781-94 (2001)
PMID: 11732897

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1023 amino acids
Theoretical weight: 116.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00722 (Residues: 10-1024; Coverage: 99%)
Gene names: JW0335, b0344, lacZ
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GTZ 4 x GTZ
Ligand MG 13 x MG
Ligand NA 11 x NA
Ligand DMS 74 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 149.69Å b: 168.4Å c: 200.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.163 0.27
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
12 more

2 mentions without citation

Intrinsic dynamic behavior of enzyme:substrate complexes govern the catalytic action of β-galactosidases across clan GH-A.
Kumar et al. (2019)
1 more