› 1jys

X-ray diffraction
1.9Å resolution

Crystal Structure of E. coli MTA/AdoHcy Nucleosidase

Released:
DOI: 10.2210/pdb1jys/pdb
PDB entry 1jys coloured by chain, front view.
PDB entry 1jys coloured by chain, side view.
PDB entry 1jys coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of E. coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase reveals similarity to the purine nucleoside phosphorylases.
Lee JE, Cornell KA, Riscoe MK, Howell PL
Structure 9 941-53 (2001)
PMID: 11591349

Function and Biology Details

Reaction catalysed: 
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 25.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AF12 (Residues: 1-232; Coverage: 100%)
Gene names: JW0155, b0159, mtn, mtnN, pfs, yadA
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand ADE 2 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21212
Unit cell:
a: 51.08Å b: 133.03Å c: 70.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.
Parveen et al. (2011)
3 more

8 mentions without citation

Prospects for the Mechanism of Spiroplasma Swimming.
Sasajima et al. (2021)
7 more