› 1jwe

Solution NMR

NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase

Released:
DOI: 10.2210/pdb1jwe/pdb
PDB entry 1jwe coloured by chain, ensemble of 20 models, front view.
PDB entry 1jwe coloured by chain, ensemble of 20 models, side view.
PDB entry 1jwe coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer.
Weigelt J, Brown SE, Miles CS, Dixon NE, Otting G
Structure 7 681-90 (1999)
PMID: 10404597

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicative DNA helicase Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACB0 (Residues: 24-137; Coverage: 24%)
Gene names: JW4012, b4052, dnaB, groP, grpA
Sequence domains: DnaB-like helicase N terminal domain
Structure domains: DNAb Helicase; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING IN TORSION ANGLE SPACE
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Electrospray ionization mass spectrometry of oligonucleotide complexes with drugs, metals, and proteins.
Beck et al. (2001)
5 more

27 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
26 more