› 1jus

X-ray diffraction
2.84Å resolution

Crystal structure of the multidrug binding transcriptional repressor QacR bound to rhodamine 6G

Released:
DOI: 10.2210/pdb1jus/pdb
PDB entry 1jus coloured by chain, front view.
PDB entry 1jus coloured by chain, side view.
PDB entry 1jus coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural mechanisms of QacR induction and multidrug recognition.
Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG
Science 294 2158-63 (2001)
PMID: 11739955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator QacR Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 194 amino acids
Theoretical weight: 23.12 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0N4 (Residues: 1-188; Coverage: 100%)
Gene names: SAP100B_005, SAP104C_022, qacR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 23 x SO4
Ligand RHQ 1 x RHQ
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P42212
Unit cell:
a: 172.3Å b: 172.3Å c: 95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.272
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
50 more

6 mentions without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)
5 more