› 1jup

X-ray diffraction
2.95Å resolution

Crystal structure of the multidrug binding transcriptional repressor QacR bound to malachite green

Released:
DOI: 10.2210/pdb1jup/pdb
PDB entry 1jup coloured by chain, front view.
PDB entry 1jup coloured by chain, side view.
PDB entry 1jup coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural mechanisms of QacR induction and multidrug recognition.
Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG
Science 294 2158-63 (2001)
PMID: 11739955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator QacR Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 194 amino acids
Theoretical weight: 22.98 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0N4 (Residues: 1-188; Coverage: 100%)
Gene names: SAP100B_005, SAP104C_022, qacR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 22 x SO4
Ligand MGR 1 x MGR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P42212
Unit cell:
a: 174.2Å b: 174.2Å c: 96.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.281
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
50 more

4 mentions without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)
3 more