› 1jko

X-ray diffraction
2.24Å resolution

Testing the Water-Mediated HIN Recombinase DNA Recognition by Systematic Mutations

Released:
DOI: 10.2210/pdb1jko/pdb
PDB entry 1jko coloured by chain, front view.
PDB entry 1jko coloured by chain, side view.
PDB entry 1jko coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Testing water-mediated DNA recognition by the Hin recombinase.
Chiu TK, Sohn C, Dickerson RE, Johnson RC
EMBO J 21 801-14 (2002)
PMID: 11847127

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114014 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-invertase hin Chain: C
Molecule details ›
Chain: C
Length: 52 amino acids
Theoretical weight: 6.05 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
UniProt:
  • Canonical: P03013 (Residues: 139-190; Coverage: 27%)
Gene names: STM2772, hin
Sequence domains: Helix-turn-helix domain of resolvase
Structure domains: Homeodomain-like
5'-D(*TP*GP*TP*TP*TP*TP*TP*GP*GP*TP*AP*AP*GP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 14 nucleotides
Theoretical weight: 4.34 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
5'-D(*AP*TP*CP*TP*TP*AP*CP*CP*AP*AP*AP*AP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 85.612Å b: 81.996Å c: 43.734Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.306
Expression system: Not provided

Quick links

Citations

1 review citation

The role of water in protein-DNA recognition.
Jayaram et al. (2004)
16 other articles

7 mentions without citation

Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization.
Fairman et al. (2011)
6 more