› 1jkk

X-ray diffraction
2.4Å resolution

2.4A X-RAY STRUCTURE OF TERNARY COMPLEX OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE WITH ATP ANALOGUE AND MG.

Released:
DOI: 10.2210/pdb1jkk/pdb
PDB entry 1jkk coloured by chain, front view.
PDB entry 1jkk coloured by chain, side view.
PDB entry 1jkk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the catalytic domain of human protein kinase associated with apoptosis and tumor suppression.
Tereshko V, Teplova M, Brunzelle J, Watterson DM, Egli M
Nat Struct Biol 8 899-907 (2001)
PMID: 11573098

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53355 (Residues: 2-295; Coverage: 21%)
Gene names: DAPK, DAPK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 46.63Å b: 62.43Å c: 88.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.239
Expression system: Escherichia coli

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Citations

26 review citations

Regulation of protein kinases; controlling activity through activation segment conformation.
Nolen et al. (2004)
25 more

8 mentions without citation

A helix scaffold for the assembly of active protein kinases.
Kornev et al. (2008)
7 more