› 1jiv

X-ray diffraction
2.07Å resolution

T4 phage BGT in complex with Mg2+ : Form II

Released:
DOI: 10.2210/pdb1jiv/pdb
PDB entry 1jiv coloured by chain, front view.
PDB entry 1jiv coloured by chain, side view.
PDB entry 1jiv coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
High resolution crystal structures of T4 phage beta-glucosyltransferase: induced fit and effect of substrate and metal binding.
Moréra S, Larivière L, Kurzeck J, Aschke-Sonnenborn U, Freemont PS, Janin J, Rüger W
J Mol Biol 311 569-77 (2001)
PMID: 11493010

Function and Biology Details

Reaction catalysed: 
Transfers a beta-D-glucosyl residue from UDP-alpha-D-glucose to a hydroxymethylcytosine residue in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA beta-glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.72 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04547 (Residues: 1-351; Coverage: 100%)
Gene name: bgt
Sequence domains: Bacteriophage T4 beta-glucosyltransferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 62.724Å b: 72.749Å c: 79.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.237
Expression system: Escherichia coli

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Citations

8 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
7 more