› 1jgl

X-ray diffraction
2.15Å resolution

Crystal structure of immunoglobulin Fab fragment complexed with 17-beta-estradiol

Released:
DOI: 10.2210/pdb1jgl/pdb
PDB entry 1jgl coloured by chain, front view.
PDB entry 1jgl coloured by chain, side view.
PDB entry 1jgl coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of a recombinant anti-estradiol Fab fragment in complex with 17beta -estradiol.
Lamminmäki U, Kankare JA
J Biol Chem 276 36687-94 (2001)
PMID: 11451948

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233687 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.67 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7TS98 (Residues: 23-236; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Ig gamma-1-chain Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand EST 1 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200HB
Spacegroup: P212121
Unit cell:
a: 48.207Å b: 64.496Å c: 165.285Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.249
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Splenic marginal-zone lymphoma: ontogeny and genetics.
Baliakas et al. (2015)
20 other articles

2 mentions without citation

Recognition of functional sites in protein structures.
Shulman-Peleg et al. (2004)
1 more